Fig. 6: PCDH17 competitively binding to the E3 ubiquitin ligase MARCH5 with VEGFR2.
From: PCDH17 induces colorectal cancer metastasis by destroying the vascular endothelial barrier
A The ubiquitinase and substrate website predicts that MARCH5 is predicted to be a ubiquitin ligase of VEGFR2 using the UbiBrowser database. B Venn diagram of the overlapping predicted E3 ligase and proteins of IP-MS. C Co-IP assay showing that PCDH17 binding to MARCH5 in HUVECs. D Co-IP assay showing that 3×Flag-tagged PCDH17 interacts with Myc-tagged MARCH5 in HEK293T cells. E Representative blots of ubiquitination of VEGFR2 in HUVECs infected with or without 3×Flag-tagged PCDH17. F Representative blots of ubiquitination of VEGFR2 in HEK293T infected with or without 3×Flag-tagged PCDH17. G Co-IP assay to detect the interaction between PCDH17, MARCH5 and VEGFR2 in HEK293T cells. H MARCH5 ubiquitinates VEGFR2 through Lys48-linked ubiquitin chains in HEK293T cells and the effects of transfection with mutant HA-ubiquitin vectors (K48R and K63R).
