Fig. 5: NEDD4 mediates PTEN ubiquitination to induce OXA resistance. | Cell Death & Disease

Fig. 5: NEDD4 mediates PTEN ubiquitination to induce OXA resistance.

From: Histone lactylation promotes multidrug resistance in hepatocellular carcinoma by forming a positive feedback loop with PTEN

Fig. 5

A Immunofluorescence confirmed the colocalization of NEDD4 and PTEN in OXA-sensitive (Ctrl) and OXA-resistant (Res) cells, scale bar = 10 µm. The X-axis represents the pixel intensity of NEDD4 (red), and the Y-axis represents the pixel intensity of PTEN (green). B Colocalization was quantified via ImageJ (n = 3). C Co-IP confirmed that NEDD4 binds to PTEN in in OXA-sensitive (Ctrl) and OXA-resistant (Res) cells. D, E Western blotting was used to detect and quantify the half-life of PTEN after treatment with CHX alone. F, G Western blot analysis revealed changes in PTEN protein levels after treatment with MG132 alone. H Detection of NEDD4 binding and ubiquitination levels using western blotting after Co-IP with PTEN in OXA-sensitive (Ctrl) and OXA-resistant (Res) cells. I, J Western blot analysis of PTEN and AKT/mTOR protein levels (n = 3). PCC Pearson’s correlation coefficient *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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