Fig. 2: SIRT2/CBP deacetylates/acetylates CLYBL at K154. | Cell Death & Disease

Fig. 2: SIRT2/CBP deacetylates/acetylates CLYBL at K154.

From: Positive feedback loop involving AMPK and CLYBL acetylation links metabolic rewiring and inflammatory responses

Fig. 2

A, B Co-immunoprecipitation assay showing the interaction of CLYBL with SIRT2 in 293 T cells. C 293 T cells treated with or without trichostatin A (TSA; 0.5 μM, 16 h) and nicotinamide (NAM; 5 mM, 4 h) to detect the acetylation of CLYBL (**p < 0.01). D Immunoprecipitation assay determining the acetyltransferase of CLYBL in 293 T cells. E, F Co-immunoprecipitation assay showing the interaction of CBP with CLYBL in 293 T cells. G 293 T cells transfected with Flag-WT-SIRT2, Flag-Q167AH187Y-SIRT2 or Flag-Vector to confirm the suppression of CLYBL acetylation by SIRT2 (**p < 0.01; ***p < 0.001). H In 293 T cells, acetylation of CLYBL was detected following treatment with or without AGK2 (20 μM, 24 h) (*p < 0.05). 293 T cells transfected with Flag-WT-CLYBL or Flag-K55R-CLYBL (Mut) (I), Flag-WT-CLYBL or Flag-K154R-CLYBL (Mut) (J), Flag-WT-CLYBL or Flag-K307R-CLYBL (Mut) (K), and with the presence or absence of Myc-SIRT2 to determine the degree of CLYBL acetylation (*p < 0.05; **p < 0.01; ns, not significant). 293 T cells were transfected with Flag-WT-CLYBL or Flag-K55R-CLYBL (Mut) (L), Flag-WT-CLYBL or Flag-K154R-CLYBL (Mut) (M), Flag-WT-CLYBL or Flag-K307R-CLYBL (Mut) (N), and in the presence or absence of Flag-CBP to determine the degree of CLYBL acetylation (*p < 0.05; ns, not significant).

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