Fig. 2: MiR-124 enhances the sensitivity of GBM cell lines to TMZ treatment. | Cell Death & Disease

Fig. 2: MiR-124 enhances the sensitivity of GBM cell lines to TMZ treatment.

From: Overexpression of miR-124 enhances the therapeutic benefit of TMZ treatment in the orthotopic GBM mice model by inhibition of DNA damage repair

Fig. 2

A, B CCK8 assay was used to assess the cell proliferative ability of different groups. U87 and U251 cells transfected with miR-Ctrl or miR-124 followed by TMZ treatment in (A), U87 and U251 cells transfected with anti-miR-Ctrl or anti-miR-124 followed by TMZ treatment in (B). Representative images of flow cytometry (C, left) and fluorescence imaging (D, left) and the variation ratio of Cherry/GFP cells (C and D, right) from co-culture cell competition assay in U87 and U251 cells exposed to 200 μM TMZ, with or without miR-124 overexpression. Scale bar, 300 μm. E PI/Annexin V-staining to detect the apoptosis of U87 and U251 cells exposed to TMZ (200 μM, 24 h) with or without miR-124 transfection (Representative images in left), Annexin V-stained (PI−/Annexin V+ and PI+/Annexin V+) cells were analyzed to measure the cell apoptosis rate (right). Above data are presented as means ± SD. All experiments were repeated at least 3 times. P values were calculated using unpaired Student’s t-test.

Back to article page