Fig. 3: MiR-124 enhances the responses of TMZ in GBM cells by blocking DNA damage repair. | Cell Death & Disease

Fig. 3: MiR-124 enhances the responses of TMZ in GBM cells by blocking DNA damage repair.

From: Overexpression of miR-124 enhances the therapeutic benefit of TMZ treatment in the orthotopic GBM mice model by inhibition of DNA damage repair

Fig. 3

A, B Representative images (left) and DNA tailing rate (right) from comet assay in U87 and A172 cells exposed to TMZ (500 μM, 4 h), with or without miR-124 overexpression. Scale bar, 200 μm (% DNA in tail: Tail DNA Intensity/Cell DNA Intensity × 100, miR-ctrl plus TMZ vs miR-124 plus TMZ, P < 0.0001). C, D Representative images (left) and quantification of RAD51 fluorescence foci (right) of immunofluorescence analysis in U87 and U251 cells exposed to TMZ (200 μM, 24 h) with or without miR-124 transfection. Scale bar, 5 μm. E, F Representative images (left) and quantification of γ-H2AX fluorescence foci (right) of immunofluorescence analysis in U87 and U251 cells exposed to TMZ (200 μM, 24 h) with or without miR-124 transfection. Scale bar, 5 μm. All experiments were repeated at least 3 times. Above data are presented as means ± SD. P values were calculated using unpaired Student’s t-test.

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