Fig. 5: A higher expression of IL-32 characterizes plasma and right ventricle of ACM patients compared to HC. | Cell Death & Disease

Fig. 5: A higher expression of IL-32 characterizes plasma and right ventricle of ACM patients compared to HC.

From: Analysis of effector/memory regulatory T cells from arrhythmogenic cardiomyopathy patients identified IL-32 as a novel player in ACM pathogenesis

Fig. 5

a, b Relative mRNA expression of total (a) and γ isoform (b) of the IL-32 gene in PBMC from ACM patients or healthy donors (HC). Data were expressed using the 2−ΔΔCt method over the housekeeping gene GAPDH. c Percentages of IL-32+ mTreg in CD4+ T cells from PBMC of ACM patients or healthy donors determined by flow cytometry after intracellular staining. d IL-32 concentration (pg/ml) in the plasma of ACM or HC (n = 24 each). e, f Representative images of right ventricular tissue sections from HC and ACM showing IL-32 staining by immunofluorescence (e) and histogram quantification (f), data are expressed as the total area of IL-32 positivity on nuclei area. Data are shown as mean +/− SEM. **p < 0.01, *p < 0.05 using T-test. Scale bar: 50 µm.

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