Fig. 6: FAM210B promoted the expression of IFN-α/β via activating IRF3 and associating with TOM70. | Cell Death & Disease

Fig. 6: FAM210B promoted the expression of IFN-α/β via activating IRF3 and associating with TOM70.

From: FAM210B activates STAT1/IRF9/IFIT3 axis by upregulating IFN-α/β expression to impede the progression of lung adenocarcinoma

Fig. 6

A Western blot assay of the expression of p-IRF3 and IRF3 in H1299 cells transfected with GFP-V or GFP-FAM210B. B Nuclear-cytoplasmic separation assays were used to analyze the expression changes of p-IRF3 in both nuclear and cytoplasmic fractions in H1299 cells transfected with GFP-V or GFP-FAM210B. C IF assay of localization of p-IRF3 in H1299 cells transfected with GFP-V or GFP-FAM210B. Scale bar: 10 μm. D qRT-PCR assays of the indicated genes in OE-V and OE-FAM210B H1299 cells combined with si-IRF3 as indicated. E Silver staining of the immunocomplex immunoprecipitated by FLAG antibody and non-immune IgG antibody. F The interaction of FLAG-FAM210B with TOM70 confirmed by co-IP assay in H1299 OE-FAM210B cells. G Imaging assay was used to assess the colocalization of GFP-FAM210B with mitochondria (MitoTracker Red) in live H1299 cells (upper), and IF assay was performed to detect the colocalization of GFP-FAM210B with TOM70 in H1299 cells transfected with GFP-FAM210B (below). Scale bar: 10 μm. H MTT assay of the cellular viability of OE-V and OE-FAM210B H1299 cells transfected with si-TOM70. I Transwell assays of the migration of OE-V and OE-FAM210B H1299 cells transfected with si-TOM70. The corresponding quantitative analysis of this transwell assay is shown in (J). K qRT-PCR assay of the expression of IFN-α/β in OE-V and OE-FAM210B H1299 cells transfected with si-TOM70. All quantitative analyses are presented as mean ± SEM from three independent experiments. Statistical analysis was conducted using Student’s t-test (D, J, K) and two-way ANOVA (H). *P < 0.05, **P < 0.01, ***P < 0.001.

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