Fig. 3: Characterization of cytosolic Ca²⁺ homeostasis in FDB fibers of WT and CrT^−/y mice.

WT and CrT^−/y myofibers were loaded with cytosolic Fura-2/AM. A Resting cytosolic Ca²⁺ concentrations; B representative traces of cytosolic Ca²⁺ concentrations upon caffeine stimulation; C cytosolic Ca²⁺ concentrations upon caffeine stimulation. Data are presented as mean ± SEM. n > 20. For data analysis, parametric Student t-test (two-tailed, unpaired) was used. ** p < 0.01. D Representative Western blot of WT and CrT^−/y TA muscles stained with α-RyR1 antibody. α-Actin was used as loading controls. n = 3. E Quantification of the immunoblots showed in D. The levels of the protein were normalized by actin levels. Data are presented as mean ± SEM. n = 3. For data analysis, parametric Student t-test (two-tailed, unpaired) was used. ** p < 0.01. F Representative Western blot of WT and CrT^−/y TA muscles stained with α-SERCA1 and α-SERCA2 antibodies. α-Actin was used as loading controls. n = 3. G Quantification of the immunoblots showed in D. The levels of the protein were normalized by actin levels. Data are presented as mean ± SEM. n = 3. For data analysis, parametric Student t-test (two-tailed, unpaired) was used. **p < 0.01.