Fig. 5: Mitochondrial Ca²⁺ uptake and membrane potential are decreased in CrT^−/y muscles compared to WT and the mRNA and protein expression of crucial components of the MCU complex is elevated.

WT and CrT^−/y myofibers were loaded with mitochondrially targeted Fura-2/AM (mitoFura-2/AM). A Resting mitochondrial Ca²⁺ concentrations are unaltered in CrT^−/y myofibers compared to WT. B Ratiometric measurements of mitochondrial Ca²⁺ uptake upon caffeine treatment in CrT^−/y FDB myofibers compared to WT fibers. C Representative traces of the experiment. Data are presented as means ± SEM. n > 20. For data analysis, parametric Student t-test (two-tailed, unpaired) was used. ***p < 0.001. D TMRM fluorescence in FDB fibers of WT and CrT^−/y mice. Data are presented as mean ± SEM. n = 20. For data analysis, parametric Student t-test (two-tailed, unpaired) was used. **p < 0.01. E Representative Western blot of the OXPHOS complexes in WT and CrT^−/y muscles. n = 3. F Relative skeletal muscle ATP levels in EDL muscles of WT and CrT^-/y muscles. n = 3. For data analysis, parametric Student t-test (two-tailed, unpaired) was used. *p < 0.05. G Real-time RT-PCR analyses of WT and CrT^−/y muscles with the indicated oligonucleotide primers. Data are presented as mean ± SEM. n = 3. For data analysis, parametric Student t-test (two-tailed, unpaired) was used. *p < 0.05. H Representative Western blot of WT and CrT^−/y EDL muscles. α-CoxIV was used as loading control. n = 4. I Real-time RT-PCR analyses of WT and CrT^−/y muscles with the indicated oligonucleotide primers. Data are presented as mean ± SEM. n = 5. For data analysis, parametric Student t-test (two-tailed, unpaired) was used. **p < 0.01. L Representative Western blot of WT and CrT^−/y TA muscles stained with α-Tom20, α-AFG3L2 and α-PHD antibodies. α-Actin was used as loading controls. n = 3.