Fig. 5: TRIM21mediates K48-linked ubiquitination degradation of PRMT1 in CRC cells.
From: TRIM21-mediated PRMT1 degradation attenuates colorectal cancer malignant progression
A TRIM21 mediated ubiquitination degradation of PRMT1 in HCT-116 and LoVo cells. B Western blot analysis showing the effect of the proteasome inhibitor MG132 (10 μM for 6 h) treatment on PRMT1 protein accumulation in HCT-116 and LoVo cells. The relative intensity of PRMT1 proteins were quantified by software Image J. C Schematic representation of TRIM21 truncated mutations. D HCT-116 cells were transfected with wild-type TRIM21 or the indicated mutants to detected the potential domain that interacted with PRMT1. E K48-only and K63-only ubiquitin plasmids alone or co-transfected with TRIM21-HA plasmid into 293 T cells for 48 h, and the cells were then harvested and subjected to myc IP.
