Fig. 6: PRMT1 is required for TRIM21-mediated CRC progression in vitro.
From: TRIM21-mediated PRMT1 degradation attenuates colorectal cancer malignant progression
A Western blot was used to detect the overexpression efficiency of TRIM21 and PRMT1 in HCT-116 cells. The relative intensity of PRMT1 proteins were quantified by software Image J. B, C Clone formation assays (B) and CCK-8 assays (C) were used to assess the effect of TRIM21 overexpression, PRMT1 overexpression or both on HCT-116 cell proliferation. D Detection of p16 and p21 expression in TRIM21 overexpression HCT116 cells after over-expressing PRMT1 or not. E, F Representative images (E) and statistical results (F) of transwell migration assays and Matrigel invasion assays to assess the migration and invasion ability of HCT-116 cells with TRIM21 overexpression, PRMT1 overexpression or both.Data are represented as mean ± SD of three independent experiments, and *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t-test).
