Fig. 8: TFF3 enhances c-MET signaling through a positive feedback loop to enhance the CSC-like phenotype of ER+HER2+ MC. | Cell Death & Disease

Fig. 8: TFF3 enhances c-MET signaling through a positive feedback loop to enhance the CSC-like phenotype of ER+HER2+ MC.

From: Inhibition of TFF3 synergizes with c-MET inhibitors to decrease the CSC-like phenotype and metastatic burden in ER+HER2+ mammary carcinoma

Fig. 8

A Western blot analysis was conducted to assess the level of c-MET and c-MET phosphorylation at Y1234/1235 in MDA-MB-361 cells with TFF3 forced expression (V & T) and depletion (sh & shT). β-ACTIN was used as input control. The sizes of detected protein blots in kDa are on the left. B Western blot analysis was conducted to assess the level of c-MET and c-MET phosphorylation at Y1234/1235 in MDA-MB-361 and BT474 cells with AMPC (0, 0.1, 0.5, 1, 5, or 10 μM) treatment for 3 days. β-ACTIN was used as input control. The sizes of detected protein blots in kDa are shown on the left. C Western blot analysis was performed to assess the level of TFF3 in MDA-MB-361 and BT474 cells transfected with scrambled siRNA, siMET#1, or siMET#2 plasmid. β-ACTIN was used as input control. The sizes of detected protein blots in kDa are shown on the left. D Western blot analysis was performed to assess the level of TFF3 in MDA-MB-361 and BT474 cells treated with Cabozantinib (0, 0.02, 0.1, 0.2, 1 or 2 μM), SU11274 (0, 0.02, 0.1, 0.2, 1 or 2 μM) or PHA-665752 (0, 0.04, 0.2, 0.4, 2 or 4 μM) for 3 days. β-ACTIN was used as input control. The sizes of detected protein blots in kDa are shown on the left. E A potential c-MET/TFF3 interaction in MDA-MB-361 cells was investigated by immunoprecipitation (IP) and immunoblotting. F MDA-MB-361 and BT474 cells transfected with scrambled siRNA, siMET#1, or siMET#2 plasmid were harvested and incubated with ALDEFLUOR substrate to define the ALDH1-positive population. DEAB was used to establish the baseline fluorescence. Data are expressed as mean ± SD (n = 3). Statistical significance is indicated as *P < 0.05, **P < 0.01, and ***P < 0.001. G Western blot analysis was utilized to examine the level of CSC-related proteins in MDA-MB-361 and BT474 cells transfected with scrambled siRNA, siMET#1, or siMET#2 plasmid. β-ACTIN was used as input control. The sizes of detected protein blots in kDa are shown on the left.

Back to article page