Fig. 3: Effect of NE on MUL1 in cultured cardiomyocytes. | Cell Death & Disease

Fig. 3: Effect of NE on MUL1 in cultured cardiomyocytes.

From: 17-beta estradiol prevents cardiac myocyte hypertrophy by regulating mitochondrial E3 ubiquitin ligase 1

Fig. 3

A Chip-atlas data representing the MACS2 pic values generated within 10 kb of the MUL1 gene transcription start site. Each dot represents a putative binding site. Primary cultured NRVMs were treated with E2 (0, 1, 10, 100 nM) for 6 h and then with NE (20 μM) for 48 h. B Protein extracts were obtained and analyzed by western blotting using a MUL1 antibody (N = 7). β-tubulin was used as a loading control. C MUL1 mRNA levels were determined by RT-qPCR (N = 4). 18S RNA was used to normalize the data. The data correspond to the mean ± SEM. Each independent experiment is displayed as a dot in the graphs. Results were analyzed using one-way ANOVA followed by multiple Tukey’s comparisons. *p < 0.05.

Back to article page