Fig. 1: Irisin reduces PrP106–126-induced neuronal apoptosis. | Cell Death & Disease

Fig. 1: Irisin reduces PrP106–126-induced neuronal apoptosis.

From: Irisin regulates oxidative stress and mitochondrial dysfunction through the UCP2-AMPK pathway in prion diseases

Fig. 1

A, B After N2a cells were treated with irisin and PrP106–126, apoptosis was assessed by Annexin V-FITC/PI flow cytometry, and cell apoptosis was measured. C, D Determination of N2a cell apoptosis by TUNEL staining, (scale bar = 50 µm). E–I Detection of cleaved caspase-3, cleaved caspase-9, Bax and Bcl2 protein expression in N2a cells by western blotting. J–L The cytochrome c protein expression in cytosolic and mitochondrial extracts in N2a cells. The data are presented as the means ± SDs (n = 6), *P < 0.05; **P < 0.01; ***P < 0.001.

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