Fig. 3: CDCA7 enhances glycolysis in PC cells and promotes gemcitabine resistance. | Cell Death & Disease

Fig. 3: CDCA7 enhances glycolysis in PC cells and promotes gemcitabine resistance.

From: CDCA7 enhances STAT3 transcriptional activity to regulate aerobic glycolysis and promote pancreatic cancer progression and gemcitabine resistance

Fig. 3

A, B Samples were stratified into high (≥ 50%) and low (< 50%) expression groups based on CDCA7 expression levels, and single-gene Gene Set Enrichment Analysis (GSEA) was conducted to identify pathways associated with CDCA7 upregulation and downregulation. C–E Measurement of lactate (C), glucose (D) and ATP (E) levels in PC cells following CDCA7 overexpression or knockdown. F, G Extracellular acidification rate (ECAR) indicates aerobic glycolysis level in PC cells following CDCA7 overexpression or knockdown. H CCK8 assay was performed detects the sensitivity of PC cells in the control, CDCA7 overexpression, and CDCA7 + 2-DG groups to gemcitabine. I Establishment of xenograft tumor model and isolation of the subcutaneous tumor 42 days later. J, K Tumor weight and volume. L IHC was performed to detect Ki-67 and PCNA in tumor tissues in each group. *P < 0.05, **P < 0.01, ***P < 0.001.

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