Fig. 4: Interaction between CDCA7 and STAT3. | Cell Death & Disease

Fig. 4: Interaction between CDCA7 and STAT3.

From: CDCA7 enhances STAT3 transcriptional activity to regulate aerobic glycolysis and promote pancreatic cancer progression and gemcitabine resistance

Fig. 4

A Detection of different protein bands using silver staining method following CDCA7 overexpression in PANC-1 cells. B Mass spectrometry analysis of five proteins coprecipitated with CDCA7. C A distinct CDCA7 polypeptide was identified from the protein lysate of anti-CDCA7 immunoprecipitated PANC-1 cells using two-dimensional LC-MS/MS mass spectrometry. D Co-immunoprecipitation assay to detect the interaction between CDCA7 and STAT3. E CDCA7 co-localizes with STAT3 in PC cells. F Design of CDCA7 and STAT3 mutants. G Immunoprecipitation and western blotting were performed to detect the interaction between flag-tagged truncated CDCA7 protein and his-tagged STAT3 protein in HEK 293 T. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page