Fig. 6: CDCA7-STAT3 axis enhances HK2 expression and promotes glycolysis. | Cell Death & Disease

Fig. 6: CDCA7-STAT3 axis enhances HK2 expression and promotes glycolysis.

From: CDCA7 enhances STAT3 transcriptional activity to regulate aerobic glycolysis and promote pancreatic cancer progression and gemcitabine resistance

Fig. 6

A Western blotting detected changes in key enzymes during glycolysis. B Correlation between CDCA7 and HK2 in the GEPIA database. C HK2 mRNA expression in PC tissue samples and normal pancreatic tissue samples using data from the GTEx and TCGA databases. D, E qRT-PCR and western blotting were performed to detect HK2 expression after CDCA7 knockdown or overexpression. F, G HK2 expression was detected in CDCA7 overexpressing cells transfected with or without sh-HK2 using qRT-PCR and western blotting. H, I Western blotting and qRT-PCR were performed detect HK2 expression following STAT3 knockdown or overexpression. J CHIP-qPCR detected STAT3 and HK2 gene. K Luciferase activity was detected PC cells co-transfected with CDCA7 and sh-STAT3. L STAT3 phosphorylation level was detected using western blotting. M Western blotting was performed to detect Whole cell lysates (WCLs) and nuclear expression of STAT3 protein. *P < 0.05, **P < 0.01, ***P < 0.001.

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