Fig. 2: USP35 regulates MAVS activity.
From: Deubiquitination enzyme USP35 negatively regulates MAVS signaling to inhibit anti-tumor immunity
A Transfection of the indicated plasmids in 293 T to detect the effect of USP35-WT and CA on the activity of IFNβ reporter gene, n = 3. B Co-transfection of the indicated plasmids in 293T to detect overexpression of USP35 for RIG-I, MAVS, and TBK1-activated IFNβ/ISRE reporter gene, n = 3. Data are shown as Mean ± s.d. Significance statistics were performed by One-way ANOVA, where **P < 0.01, ns, no statistical difference, and n represents the number of experimental replicates. C In B16F10, a stable USP35 knockdown cell line was generated using shRNA. Cells were collected at 0 h and 6 h post-VSV virus infection, and qRT-PCR was performed to measure the mRNA levels of IFNβ, CXCL10, and ISG15. N = 3. D–F In the Yummer1.7, HeLa, and ID8 with knockdown of USP35, the levels of IFN β, CXCL10, and ISG 15 were measured by qRT-PCR after infection with VSV virus, n = 3. The data show that all are Mean ± s.d, the significance statistics method is One-way ANOVA, where *P < 0.05, **P < 0.01, ns is not statistically different, and n is the number of experimental replicates. G mRNA expression levels of IFNβ, CXCL10, and ISG15 were detected by qRT-PCR at 24 h after 3pRNA transfection in B16F10. N = 3. Data are shown as Mean ± s.d. Significance statistics were performed by One-way ANOVA, where *P < 0.05, ns not statistically different. n is the number of replicates of the experiment.
