Fig. 3: USP35 interacts with MAVS.
From: Deubiquitination enzyme USP35 negatively regulates MAVS signaling to inhibit anti-tumor immunity
A Co-transfection of Myc-USP35 with the indicated constructs in 293T. The binding of USP35 to RIGI, TBK1, cGAS, and MAVS was detected by co-immunoprecipitation (co-IP). B 293T were transfected with Myc-USP35 and Flag-MAVS. Co-IP was performed to detect the interaction between USP35 and MAVS. C Overexpression of the indicated plasmids in 293T were infected with 0.5 MOI VSV virus for the indicated time, and then the lysates were subjected to co-IP. D The interaction between endogenous USP35 and MAVS in ID8 was examined by co-IP experiment using anti-USP35 antibody. E The interaction between endogenous USP35 and MAVS in ID8 was examined by co-IP experiment using anti-MAVS antibody. F GST pull-down assay was performed using purified USP35 protein with Flag-tagged MAVS expressed in 293T. G 293T transfected with the indicated constructs were subjected to co-IP and western blot analysis. Schematic illustration of the primary structure of MAVS is shown. H 293T transfected with the indicated constructs were subjected to co-IP and western blot analysis. Schematic illustration of the primary structure of USP35 is shown.
