Fig. 5: USP35 regulates the MAVS-TBK1-IRF3 signaling pathway.
From: Deubiquitination enzyme USP35 negatively regulates MAVS signaling to inhibit anti-tumor immunity
A, B HeLa, Yummer1.7 infected with VSV (0, 3, 6, and 9 h) in knockdown of USP35 and control groups were assayed for pTBK1, pIRF3, TBK1, IRF3 and GAPDH by western blot. C, D, F VSV infected with knockdown of USP35 and control groups of ID8, Yummer 1.7, A375 (0, 3, 6, and 9 h), and protein levels of pTBK1, pIRF3, as well as TBK1, IRF3, and GAPDH were detected by Western Blot. E USP35 was knocked out in B16F10cas9 and the knockout of USP35 and control groups were infected with VSV (0, 3, 6, and 9 h), and the protein expression of p-TBK1, p-IRF3, and GAPDH, TBK1, and IRF3 were detected by WB.
