Fig. 6: USP35 regulates immune cell infiltration in malignant melanoma and enhances the sensitivity to oncolytic virotherapy.

A When the tumors in mice reached approximately 6 mm in size, they were injected intratumorally with tumor Oncolytic virus H101 or PBS (d12, d15, d18, d21, d23), respectively, according to the corresponding groups; H101: 1.5 × 10⁹ vp was diluted to 50 μl, while the control group received an equal volume of PBS. Eight mice were used per group. B, C When tumor length and diameter in control mice reached approximately 15 mm, the mice were executed for weighing and photographing according to groups. D Tumor growth was monitored by measuring the long and short diameters of tumors every 2 days. Tumor volume was calculated using the formula: volume (mm³) = (long diameter \(\times\) short diameter²)/2. Tumor growth curves were plotted and subjected to statistical analysis. E In the B16F10 mouse model, the percentages of positive CD4+ and CD8⁺ T cells were measured in the different experimental groups. F The proportion of CD8⁺ T cells positive for infiltration in each group. G In the B16F10 mouse model, the percentages of positive F4/80⁺ and CD206⁺ macrophages were measured in the different experimental groups. H The proportion of CD206⁺ macrophages in each group.