Fig. 2: DMF suppresses cell proliferation and decreases HNF1B protein level. | Cell Death & Disease

Fig. 2: DMF suppresses cell proliferation and decreases HNF1B protein level.

From: Dimethyl fumarate promotes the degradation of HNF1B and suppresses the progression of clear cell renal cell carcinoma

Fig. 2

A CCK-8 assays of the proliferation of 786-O and RCC4 cells treated with a serial dose of DMF as indicated. The data represent the mean ± SD of n = 3 independent experiments. Statistical differences were determined by one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001. B Colony formation assays and statistical analysis of the proliferation of 786-O and RCC4 cells treated with a serial dose of DMF as indicated. The data represent the mean ± SD of n = 3 independent experiments. Statistical differences were determined by one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ***P < 0.0001. C Western blot analysis of 786-O and RCC4 cells stably overexpressing Flag-HNF1B. D Western blot analysis of exogenous Flag-HNF1B in 786-O and RCC4 cells treated with or without DMF (50 μM, 12 h). E Western blot analysis of endogenous HNF1B in 786-O and RCC4 cells treated with or without DMF (50 μM, 12 h). F Western blot analysis of HNF1B treated with 50 μM DMF for different times as indicated. G Western blot analysis of HNF1B treated with increasing concentrations of DMF (0 to 100 μM) for 12 h. qPCR analysis of CRB3, KIF12 and PKHD1 levels in 786-O (H) and RCC4 (I) cells treated with or without 50 μM DMF for 12 h.

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