Fig. 4: HNF1B succination increases its ubiquitination. | Cell Death & Disease

Fig. 4: HNF1B succination increases its ubiquitination.

From: Dimethyl fumarate promotes the degradation of HNF1B and suppresses the progression of clear cell renal cell carcinoma

Fig. 4

A Co-IP analysis of Flag-HNF1B succination in HEK-293T cells transfected with Flag-HNF1B with or without DMF treatment. B The effect of N-acetyl-L-cysteine (NAC) on DMF-induced HNF1B degradation was determined by western blot. Cells were pretreated with 5 mM NAC for 1 h before treated with DMF (50 μM, 12 h). C Western blot analysis of Flag-HNF1B derived from 786-O cells stably expressing WT, C223S, C268S, C273S, C552S or 4CS of HNF1B treated with or without DMF (50 μM, 12 h). D Succination of WT or 4CS mutant of HNF1B were analyzed by co-IP assay in HEK-293T cells transfected with indicated plasmids. E The effects of DMF on the interaction between HNF1B WT or 4CS mutant and HA-OTUD3 were determined by co-IP and western blot. F HEK-293T cells were transfected with HA-Ub and Flag-HNF1B WT or 4CS mutant in the presence or absence of DMF. Ubiquitination levels of WT HNF1B and 4CS mutant were analyzed by co-IP and western blot.

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