Fig. 4: The m6A methylation of MEG3 mediated by METTL3 plays a role in radiation-induced hepatocyte injury. | Cell Death & Disease

Fig. 4: The m6A methylation of MEG3 mediated by METTL3 plays a role in radiation-induced hepatocyte injury.

From: The YTHDC1 reader protein recognizes and regulates the lncRNA MEG3 following its METTL3-mediated m6A methylation: a novel mechanism early during radiation-induced liver injury

Fig. 4

A Predictive analysis of MEG3-m6A methylation sites. B Predictive analysis of putative binding sites between MEG3 and YTHDC1. C Through the combination of results from (A, B), YTHDC1-dependent methylation sites were identified. D Confirmation of the successful knockdown of METTL3 in BNL CL2 cells (**P < 0.01). qPCR was used to detect levels of MEG3 in cells following METTL3 knockdown without (E) or with (F) 6 Gy irradiation (**P < 0.01). MeRIP was used to detect MEG3 methylation levels in BNL CL2 cells in which METTL3 was knocked down without (G) or with (H) 6 Gy irradiation (**P < 0.01). I MEG3 methylation levels were detected via MeRIP assay in BNL CL2 cells at 2 h following 6 Gy irradiation (**P < 0.01). J Overview of the MeRIP experimental approach. D–I n = 3. D–I were performed by two-tailed unpaired T-test.

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