Fig. 6: MEG3 regulates radiation-induced injury in BNL CL2 cells via regulating the miR-20b-mediated control of BNIP2 expression. | Cell Death & Disease

Fig. 6: MEG3 regulates radiation-induced injury in BNL CL2 cells via regulating the miR-20b-mediated control of BNIP2 expression.

From: The YTHDC1 reader protein recognizes and regulates the lncRNA MEG3 following its METTL3-mediated m6A methylation: a novel mechanism early during radiation-induced liver injury

Fig. 6

A A dual-luciferase reporter assay was used to probe the impact of miR-20b on wild-type or mutant MEG3 reporter activity (**P < 0.01; NS no significance); The effects of irradiation (6 Gy) on miR-20b levels were assessed following the overexpression (B) or knockdown (C) of MEG3 (**P < 0.01). The impact of overexpressing MEG3 on BNIP2 mRNA and protein levels without (D) or with (E) irradiation (**P < 0.01). The impact of knocking down MEG3 on BNIP2 mRNA levels without (F) or with (G) irradiation. H The impact of knocking down MEG3 on BNIP2 protein levels in the presence or absence of irradiation (**P < 0.01, NS, no significance); MEG3 is capable of reversing miR-20b-mediated BNIP2 downregulation in the absence (I) or presence (J) of irradiation (**P < 0.01). B–J n = 3. B–G were performed by two-tailed unpaired T-test. H–J were followed by one-way ANOVA.

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