Fig. 7: miR-20b protects against radiation-induced injury in BNL CL2 cells through targeting BNIP2. | Cell Death & Disease

Fig. 7: miR-20b protects against radiation-induced injury in BNL CL2 cells through targeting BNIP2.

From: The YTHDC1 reader protein recognizes and regulates the lncRNA MEG3 following its METTL3-mediated m6A methylation: a novel mechanism early during radiation-induced liver injury

Fig. 7

A A dual-luciferase reporter assay was used to probe the impact of miR-20b on the activity of wild-type or mutant BNIP2 (**P < 0.01; NS, no significance). The impact of overexpressing miR-20b on BNIP2 mRNA and protein levels within BNL CL2 cells without (B) or with (C) irradiation. (**P < 0.01). D The impact of knocking down miR-20b on the mRNA and protein levels of BNIP2 in BNL CL2 cells that were or were not irradiated (**P < 0.01, NS no significance). E A CCK-8 assay was used to assess the effects of miR-20b inhibition on BNL CL2 cell viability following miR-20b inhibition and irradiation (**P < 0.01). F Hoechst staining was used to assess the impact of miR-20b inhibition on BNL CL2 cell apoptosis following irradiation. B–D, n = 3; A, E, n = 5; scale bar: 20 µm. B–E were performed by two-tailed unpaired T-test. D was also followed by one-way ANOVA.

Back to article page