Fig. 8: YTHDC1 plays a role in RILI in mice through the identification of m6A-modified MEG3. | Cell Death & Disease

Fig. 8: YTHDC1 plays a role in RILI in mice through the identification of m6A-modified MEG3.

From: The YTHDC1 reader protein recognizes and regulates the lncRNA MEG3 following its METTL3-mediated m6A methylation: a novel mechanism early during radiation-induced liver injury

Fig. 8

A Schematic overview of the preparation of a YTHDC1-knockdown mouse model. B Hepatic MEG3 methylation modification levels were measured following YTHDC1 knockdown in mice without or with radiation (**P < 0.01). C The impact of knocking down YTHDC1 on hepatic YTHDC1 protein levels was assessed in mice that were or were not irradiated (**P < 0.01) D The impact of silencing YTHDC1 on the hepatic protein levels of METTL3 was assessed in mice that were or were not irradiated (**P < 0.01); E. The impact of silencing YTHDC1 on the hepatic levels of miR-20b was assessed in mice that were or were not irradiated (**P < 0.01); F The impact of silencing YTHDC1 on the hepatic MEG3 levels was assessed in mice that were or were not irradiated (**P < 0.01); G The effects of silencing YTHDC1 on BNIP2 protein in the liver of mice that were or were not irradiated (**P < 0.01); H Pathological changes in murine liver tissue were assessed via H&E staining following the knockdown of YTHDC1 and irradiation-induced damage. B–G n = 8; scale bar: 20 µm. B was performed by two-tailed unpaired T-test. C–G were followed by one-way ANOVA.

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