Fig. 6: Vancomycin treatment alters resistance to C. rodentium in Atg7 mutant and WT mice.
From: Inhibition of Atg7 in intestinal epithelial cells drives resistance against Citrobacter rodentium
a Body weight changes of WT and Atg7ΔIEC mice infected with C. rodentium after vancomycin treatment (n = 5 WT mice, n = 4 Atg7ΔIEC mice, n = 4 WT+vanco mice, n = 3 Atg7ΔIEC+vanco mice). Two-way ANOVA with Greenhouse-Geisser correction. b Quantification of bacterial burden in stool (CFU/g) at day 8 after C. rodentium infection from WT and Atg7ΔIEC mice non-treated and vancomycin-treated (n = 4 WT, n = 4 Atg7ΔIEC, n = 5 WT + vanco, n = 4 Atg7ΔIEC + vanco). c Relative quantification of colon weight/length ratio from WT and Atg7ΔIEC mice non-treated and vancomycin-treated at day 18 p.i (n = 4 WT mice, n = 4 Atg7ΔIEC mice, n = 5 WT + vanco mice, n = 3 Atg7ΔIEC+vanco mice). d Representative hematoxylin & eosin (H&E), Ki67, alcian blue (BA), CD3, and CD4 staining of colonic sections from WT and Atg7ΔIEC mice non-treated and vancomycin-treated at day 18 p.i. Quantification of colonic crypts length (n = 6 WT mice, n = 3 Atg7ΔIEC mice, n = 5 WT + vanco mice and n = 3 Atg7ΔIEC+vanco mice). Quantification of Ki67 positive cells per crypts (n = 5 WT mice, n = 4 Atg7ΔIEC mice, n = 5 WT + vanco mice and n = 3 Atg7ΔIEC + vanco mice). Quantification of BA-positive cells per colonic section (n = 5 WT mice, n = 4 Atg7ΔIEC mice, n = 5 WT + vanco mice and n = 3 Atg7ΔIEC + vanco mice). Quantification of CD3 positive cells per crypt (n = 4 WT mice, n = 4 Atg7ΔIEC mice, n = 5 WT + vanco mice and n = 3 Atg7ΔIEC + vanco mice). Quantification of CD4 positive cells per colonic section (n = 6 WT mice, n = 4 Atg7ΔIEC mice, n = 5 WT+vanco mice, n = 3 Atg7ΔIEC + vanco mice). e Representative IHC staining for CD4 and Foxp3 on colonic sections from non-treated and vancomycin-treated WT and Atg7ΔIEC mice at day 18 p.i. Quantification of CD4 and Foxp3 double-positive cells per colonic sections (n = 4 WT mice, n = 4 Atg7ΔIEC mice, n = 5 WT+vanco differences: *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant, determined by unpaired t-test.
