Fig. 1: ERα interacts with several RNAs in MCF-7 BC cell nuclei. | Cell Death & Disease

Fig. 1: ERα interacts with several RNAs in MCF-7 BC cell nuclei.

From: LncRNA PVT1 links estrogen receptor alpha and the polycomb repressive complex 2 in suppression of pro-apoptotic genes in hormone-responsive breast cancer

Fig. 1

A WB showing BAZ1B, EZH2 and MEN1 enrichment following ERα immunoprecipitation with or without RNase treatment in MCF-7 nuclear extracts. IgG was used as negative control. B Scatter plot (left) showing enriched RNAs (log2 + 2) identified through ERα nuclear RIP-Seq. IgG was used as negative control. Pie chart (right) represents the enriched RNAs categories (protein coding in blue and non-coding in red). C Donut chart showing non-coding categories with different red shades. D Venn diagram (left) showing MCF-7 fitness lncRNAs among those interacting with ERα. Heatmap (right) showing screen score values of the three lncRNAs FGD5-AS1, EPB41L4A-AS1 and PVT1 selected for our investigation. Each row represents one lncRNA while the columns represent values observed in MCF-7 (left) and in ERα-negative MDA-MB-231 (right). Scale bar (up) represents the screen score (genes are considered fitness starting from a threshold screen score of 7). E RIP coupled to RT-qPCR validating the enrichment of ERα-associated lncRNAs in MCF-7 cell nuclei using IgG as negative control. lncRNA PRNCR1, selected among not enriched molecules, was used as negative control. The results showed are the mean ± SD of triplicate values. Asterisks indicate statistically significant differences using unpaired t-test (*p < 0.05 and ***p < 0.005).

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