Fig. 3: Functional portrait of FGD5-AS1, EPB41L4A-AS1 and PVT1 following ASO-mediated silencing. | Cell Death & Disease

Fig. 3: Functional portrait of FGD5-AS1, EPB41L4A-AS1 and PVT1 following ASO-mediated silencing.

From: LncRNA PVT1 links estrogen receptor alpha and the polycomb repressive complex 2 in suppression of pro-apoptotic genes in hormone-responsive breast cancer

Fig. 3

A Volcano Plots (left) displaying differentially expressed genes (green down, red up and blue common among all comparisons) identified following 72 h of ASO-mediated lncRNAs silencing. Gray dots represent not statistically significant genes (−1.5 ≥ Fold change ≤ 1.5). The Venn diagram (right) shows the intersection between ERα binding sites [30] and the common differentially expressed genes identified after lncRNAs silencing (hypergeometric test p-value < 1.81*10−3). B Dot plot of the most significant IPA canonical pathways involving common differentially expressed genes identified after silencing. Dot color ranges from red to blue depending on the –log of the p-value. Dot sizes depend on the Gene Ratio as reported in the figure. C Proliferation rate assessed by using MTT assay in MCF-7, T-47D, ZR-75-1, MDA-MB-231 and MCF-10A cells after 72 h of lncRNA silencing. Data are presented as the mean ± SD of multiple results from a representative experiment performed in multiple replicates. Results were displayed as percentage to NC and asterisks indicate statistically significant differences using unpaired t-test (*p < 0.05, **p < 0.01, ***p < 0.005 and ****p < 0.001). D WB (upper panels) and relative densitometry (lower panels) showing ERα protein level following 72 h of FGD5-AS1, EPB41L4A-AS1 and PVT1 silencing or treatment with ICI (1 μM) in MCF-7, T-47D and ZR-75-1 cell lines. Tubulin (TUB) was used as loading control. Images were processed with ImageJ software (https://imagej.net) for densitometry readings. E Heatmap showing ERα trans-activating activity assessed in MCF-7 cells stably expressing the ERE-Luc reporter gene following lncRNAs silencing or treatment with ICI (1 μM) all in presence/absence of estrogen stimuli (10 nM). All data were analyzed with respect to the scramble NC in basal condition. Data are presented as the mean of determinations from a representative experiment performed in four independent replicates after 72 h of silencing.

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