Fig. 2: Knockdown of CENPF inhibits colorectal cancer cell migration, invasion in vitro and in vivo.
From: USP4-mediated CENPF deubiquitylation regulated tumor metastasis in colorectal cancer
A Transwell assays were performed to evaluate migration and invasion ability of following CENPF knockdown in DLD1, HCT116, SW480 cells. Scale bars, 200 μm. The experiment was repeated three times. One-way ANOVA was used to compare the differences in the number of cells invading through the migration chamber in different groups. **p < 0.01; ***p < 0.001. B Representative images of the wound-healing migration assays in CENPF-knockdown DLD1 and SW480 cells compare with shCtrl cells. Scale bars, 200 μm. One-way ANOVA was used to compare the differences in the wound healing area at different time points. **p < 0.01, ***p < 0.001. C, D Representative photographs and quantification of metastatic tumor nodes in mouse livers after spleen injection (intrahepatic metastases were marked with yellow arrows). Differences in liver lesions among groups were analyzed using one-way ANOVA test. *p < 0.05, **p < 0.01. E Kaplan–Meier curves showing overall survival among different groups of mice (10 mouse models in each group). Log-rank test was used to calculate p-values compared with the shCtrl group. *p < 0.05; **p < 0.01.
