Fig. 1: circABCC4 is overexpressed in CAFs and is associated with Oxaliplatin chemoresistance and poor survival in advanced PDAC. | Cell Death & Disease

Fig. 1: circABCC4 is overexpressed in CAFs and is associated with Oxaliplatin chemoresistance and poor survival in advanced PDAC.

From: Glycolysis reprogramming in CAFs promotes oxaliplatin resistance in pancreatic cancer through circABCC4 mediated PKM2 nuclear translocation

Fig. 1

A Schematic illustration of the identification of circABCC4 upregulated in CAFs. B qRT–PCR analysis of circABCC4 expression in NFs, CAFs, primary cancer cell and PDAC cell lines. C Comparative analysis of circABCC4 expression in CAFs obtained from oxaliplatin-resistant (OXA-R) and -sensitive (OXA-S) pancreatic cancer patients via qRT–PCR (n = 3). D qRT-PCR evaluation of circABCC4 expression in OXA-S (n = 21) and OXA-R (n = 54) PDAC samples, depicted as violin plots. E, F Kaplan–Meier survival analysis for advanced PDAC patients receiving oxaliplatin-based (E) or non-oxaliplatin chemotherapy (F), stratified by high or low circABCC4 expression. G Genomic loci diagram depicting the exonic origin (11 exons) of circABCC4. H Validation of the back-splice junction of circABCC4 using Sanger sequencing. I Amplification of cDNA and gDNA in CAFs using convergent and divergent primers, with GAPDH as a negative control. J Comparative PCR analysis of circABCC4, ABCC4 and GAPDH expression in CAFs following RNase R treatment. Data are mean ± SD. ns, not significant. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. K Temporal analysis of circABCC4 and ABCC4 mRNA expression in CAFs post-treatment with actinomycin (D). L Representative FISH images for circABCC4 in CAF1 and CAF2. Scale bars, 20 μm. M Subcellular fractionation assays of circABCC4 in CAF1 and CAF2. Data are expressed as the mean ± SD. ***p < 0.001.

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