Fig. 2: circABCC4 is critical for CAFs to induce oxaliplatin resistance in PDAC cells. | Cell Death & Disease

Fig. 2: circABCC4 is critical for CAFs to induce oxaliplatin resistance in PDAC cells.

From: Glycolysis reprogramming in CAFs promotes oxaliplatin resistance in pancreatic cancer through circABCC4 mediated PKM2 nuclear translocation

Fig. 2

Panc-1 and MiaPaCa-2 cells were cocultured with CAFs stably transfected with empty vector, circABCC4, lenti-NC-shRNA, or lenti-circABCC4-shRNA for 3 days and then subjected to the indicated experiments. A–C Oxaliplatin IC50 of Panc-1 and MiaPaCa-2 determined by constructing a dose–response curve. D–G Colony formation assays of Panc-1 and MiaPaCa-2 cells without or under oxaliplatin treatment (2 μM and 4 μM). H–K Flow cytometry analysis of oxaliplatin-induced (30 μM) apoptosis in Panc-1 and MiaPaCa-2 cells. Data are expressed as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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