Fig. 9: CD44 promotes tubular cell injury and AKI partially through ferroptosis. | Cell Death & Disease

Fig. 9: CD44 promotes tubular cell injury and AKI partially through ferroptosis.

From: Tubular CD44 plays a key role in aggravating AKI through NF-κB p65-mediated mitochondrial dysfunction

Fig. 9

A HKC-8 was transfected with Ctrl-shR or CD44-shR plasmid and then treated with 5 μM erastin for 24 h. Representative micrographs show the Fe2+ content via FerroOrange staining in different groups, as indicated. Scale bar, 25 μm. B and C Quantitative results of QPCR showing relative (B) GPX4 and (C) ACSL4 mRNA levels among different groups. **P < 0.01 versus Ctrl-shR group; #P < 0.05 versus erastin with ctrl-shR (n = 3). D HKC-8 was transfected with pcDNA3 or pHA-CD44 overexpression plasmid and then treated with 5 μM erastin for 24 h. Representative micrographs show the Fe2+ content via FerroOrange staining in different groups, as indicated. Scale bar, 25 μm. E and F Quantitative result of QPCR showing relative (E) GPX4 and (F) ACSL4 mRNA levels among different groups. ***P < 0.001 versus pcDNA3 group; #P < 0.05 versus erastin with pcDNA3 (n = 3). G Representative micrographs show the Fe2+ content via FerroOrange staining in different groups, as indicated. Scale bar, 25 μm. H and I Quantitative result of QPCR showing relative H GPX4 and I ACSL4 mRNA level among different groups. ***P < 0.001 versus Ctrl-shR group; ##P < 0.01, ###P < 0.001 versus H/R with ctrl-shR (n = 3). J Quantitative result showing MDA content among different groups. ***P < 0.001 versus Ctrl-shR group; #P < 0.05 versus H/R with ctrl-shR (n = 3). K and L Quantitative result of QPCR showing relative (K) GPX4 and (L) ACSL4 mRNA levels among different groups. ***P < 0.001 versus pcDNA3 group; #P < 0.05 versus H/R with pcDNA3 (n = 3). M Quantitative result showing MDA content among different groups. ***P < 0.001 versus pcDNA3 group; #P < 0.05 versus H/R with pcDNA3 (n = 3). N Representative micrographs show the Fe2+ content via FerroOrange staining in different groups, as indicated. Scale bar, 25 μm. O Quantitative result showing total iron content in kidney tissue among different groups. ***P < 0.001 versus wild-type mice upon sham group; ##P < 0.01 versus wild-type mice upon IRI group (n = 5). P and Q Quantitative result of QPCR showing relative P GPX4 and Q ACSL4 mRNA levels among different groups. **P < 0.01, ***P < 0.001 versus wild-type mice upon sham group; #P < 0.05, ##P < 0.01 versus wild-type mice upon IRI group (n = 5). R Quantitative result showing MDA content in kidney tissue among different groups. **P < 0.01 versus wild-type mice upon sham group; #P < 0.05 versus wild-type mice upon IRI group (n = 5). S Quantitative result showing total iron content in kidney tissue among different groups. ***P < 0.001 versus sham group; ##P < 0.01 versus IRI group injected with pcDNA3 (n = 5). T and U Quantitative result of QPCR showing relative S GPX4 and T ACSL4 mRNA level among different groups. ***P < 0.001 versus sham group; #P < 0.05 versus IRI group injected with pcDNA3 (n = 5). V Quantitative result showing MDA content in kidney tissue among different groups. ***P < 0.001 versus sham group; ###P < 0.001 versus IRI group injected with pcDNA3 (n = 5).

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