Fig. 1: Immunoblot detection of ACBP/DBI in mouse tissues. | Cell Death & Disease

Fig. 1: Immunoblot detection of ACBP/DBI in mouse tissues.

From: Atlas of expression of acyl CoA binding protein/diazepam binding inhibitor (ACBP/DBI) in human and mouse

Fig. 1

A The 13 kDa band corresponding to ACBP/DBI is detected in Hep55.1C cells transiently transfected with control unrelated small interfering RNAs (siUNR) and vanished when knocking down Dbi expression with Dbi-specific small interfering RNAs (siDbi). Beta-actin is showed as loading control. The images are from 1 experiment and representative of 2 experiments. B Immunoblots of ACBP/DBI in liver tissues from female and male mice (N = 5 per group). A protein loading control (LC) was loaded at 2 µg (LC2) or 20 µg (LC20) for relative normalization between membranes. C Heatmap of the log2-scaled normalized ACBP/DBI protein expression across female and male mouse tissues. Unpaired two-sided Student t-test with Benjamini–Hochberg correction for multiple comparison was used to test for significance. .P < 0.1.

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