Fig. 3: LONP1 regulates the metabolic switch from oxidative phosphorylation to aerobic glycolysis in PCa cells.
From: Lon protease 1-mediated metabolic reprogramming promotes the progression of prostate cancer
A RNA-sequencing on LONP1-knockdown PC3 cells and GFP-LONP1 expressing DU145 cells. Heatmap showing the intersection of upregulated genes from GFP-LONP1 expressing DU145 cells and downregulated genes from LONP1- knockdown PC3 cells. The red-highlighted gene names have been previously reported to be associated with LONP1 function or implicated in cancer progression. B Cluster analysis of differentially expressed genes determined by Metascape database (www.metascape.org). C, D LONP1-knockdown PC3 cells and GFP-LONP1 expressing DU145 cells were assayed for lactate excretion (C) and ATP production (D). E, G LONP1-knockdown PC3 cells (E) and GFP-LONP1 expressing DU145 cells (G) were supplied with 10 mM glucose, 2 μM oligomycin, and 50 mM 2-DG at the indicated times. Left: ECAR was examined using a Seahorse XFe96 analyzer. Right: the glycolysis stress test parameters were automatically calculated using the Seahorse XF Glycolysis Stress Test Report Generator. F, H LONP1-knockdown PC3 cells (F) and GFP-LONP1 expressing DU145 cells (H) were supplied with 2 μM oligomycin, 2 μM FCCP, and 0.5 μM Rotenone/Antimycin A at the indicated times. Left: OCR was examined using a Seahorse XFe96 analyzer. Right: basal respiration, maximal respiration, ATP production, and spare respiratory capacity were automatically calculated using the Seahorse XF Mitochondria Stress Test Report Generator. Data are represented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.
