Fig. 2: NF-κB induced the expression of EAAT3 in vitro and in vivo.
From: NF-κB-mediated EAAT3 upregulation in antioxidant defense and ferroptosis sensitivity in lung cancer
A Eaat3 expression in mouse lung normal epithelial cell MTEC and MTEC-KO-Gprc5a cells treated with TNFα (10 ng/mL) and BMS 345541 (10 μM). B EAAT3 mRNA expression in human normal lung 16HBE epithelial cells treated with TNFα (10 ng/mL). C EAAT3 mRNA expression in human normal lung HBEC epithelial cells treated with TNFα (10 ng/mL). D EAAT3 expression in lung cancer A549 cell line treated with NF-κB inhibitor BMS 345541 (5, 10, and 15 μM) and QNZ-EVP4593(5, 10 μM). E,F EAAT3 mRNA expression in A549 cells treated with BMS (5, 10, and 15 μM) and QNZ-EVP4593(5 and 10 μM). G P65 and EAAT3 expression in A549 and PC9 cells transfected with siRNA-P65. H,I The expression of Eaat3 in Gprc5a−/− and Gprc5a−/−/SPC-dnIκBα mouse lung tissues by IHC (x10). J The expression of Eaat3 in Gprc5a−/− and Gprc5a−/−/SPC-dnIκBα mouse lung tissues by western blot (n = 6). K The expression of Eaat3 in Gprc5a−/− and Gprc5a−/−/SPC-dnIκBα mouse lung tissues by real-time PCR (n = 8). *p < 0.05, **p < 0.01, error bars represent SEM.
