Fig. 5: ONECUT3 regulates HIF-1α transcriptional activity through HDAC6. | Cell Death & Disease

Fig. 5: ONECUT3 regulates HIF-1α transcriptional activity through HDAC6.

From: Transcription factor ONECUT3 regulates HDAC6/HIF-1α activity to promote the Warburg effect and tumor growth in colorectal cancer

Fig. 5

A Volcano plot showed differentially expressed genes (DEGs) caused by ONECUT3 knockdown. B Five genes (NAA10, HNF4A, TCEB2, HDAC6 and ELAVL1) were identified after intersection of proteins interacting with HIF-1α and ONECUT3-regulated genes (n = 438). C qRT-PCR results showed the expression of HDAC6 after knockdown of ONECUT3 in HT29 and LOVO cells. D Western Blot results showed protein expression of HDAC6 after ONECUT3 knockdown. E ChIP-PCR analysis revealed that ONECUT3 was specifically bound to the HDAC6 promoter region at −1158, while no significant binding was observed at the -93 region or Exon 2, the latter serving as a negative control. F Luciferase reporter gene assay demonstrated that ONECUT3 transcriptionally regulated the HDAC6 gene. *p < 0.05, **p < 0.01, ns: no significance.

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