Fig. 6: MAPK1 attenuates miR-769-5p-mediated chemotherapy resistance and stemness.

A A Venn diagram of predicting miR-769-5p target genes using four databases, miRDB, miRBase, TargetScan and DIANA. B Protein expression of MAPK1 in antimiR-NC, antimiR-769-5p, mimics-NC, mimics-769-5p-treated HCT-8 and DLD1 cells was detected by western blot. C Protein expression of MAPK1 after co-incubation of M0-exos and GRP78-exos with HCT-8 and DLD1 cells was detected by western blot. D The mRNA expression of MAPK1 in antimiR-NC, antimiR-769-5p, mimics-NC, mimics-769-5p, M0-exos, and GRP78-exos-treated HCT-8 and DLD1 cells was detected by qRT-PCR. E The binding site of the 3’-UTR region of MAPK1 to miR-769-5p was predicted by the TargetScan database and the mutant sequences constructed from the site. F The effects of mimics-NC, mimics-miR-769-5p on pmirGLO-NC, pmirGLO-MAPK1-3’-UTR-WT, pmirGLO-MAPK1-3’-UTR-MUT luciferase activities in HCT-8 and DLD1 cells. G The effects of transfection of control, miR-769-5p, miR-769-5p/MAPK1 in HCT-8, DLD1 cells on cell survival was detected by MTT assay. H The effects of transfection of control, miR-769-5p, and miR-769-5p/MAPK1 on the protein expression of stemness markers CD133, CD44, and ALDH1A1 were detected by western blot in HCT-8 and DLD1 cells. I The effects of transfection of control, miR-769-5p, and miR-769-5p/MAPK1 on mRNA expression of stemness markers CD133, CD44, and ALDH1A1 in HCT-8 and DLD1 cells were detected by qRT-PCR. J Representative images of MAPK1 immunohistochemical staining of mouse tumor tissue in Fig. 3. Scale bar: 100 μm. K Statistical plot of positive cells in immunohistochemical staining for (J). Data are expressed as SD ± mean. *p < 0.05, **p < 0.01, ***p < 0.001.