Fig. 7: MiR-769-5p induces CRC cells into quiescence by downregulating MAPK1. | Cell Death & Disease

Fig. 7: MiR-769-5p induces CRC cells into quiescence by downregulating MAPK1.

From: MiR-769-5p of macrophage exosomes induced by GRP78 promotes stemness and chemoresistance in colorectal cancer

Fig. 7

A Schematic representation of the cell cycle in HCT-8, DLD1 cells transfected with control, miR-769-5p, miR-769-5p/MAPK1 as detected by flow cytometry. B Statistical plots of the proportion of cells in the G0/G1, S, and G2/M phases for the (A). C Edu-stained fluorescence images of control, miR-769-5p, miR-769-5p/MAPK1 transfected in HCT-8, DLD1 cells. Scale bar: 10 μm. D Statistical plot of Edu positive cells in (C). E Changes in the ki-67lowp27high cell population after transfection with control, miR-769-5p, and miR-769-5p/MAPK1 were detected by flow cytometry in HCT-8 and DLD1 cells. Red numbers represent the percentage of this cell population. F Statistical plot of the percentage of ki-67lowp27high cell populations in (E). G The effects of transfection of control, miR-769-5p, miR-769-5p/MAPK1 on the protein expression of cycle regulatory proteins RB1, p-RB1, cyclin E1, and cyclin D1 in HCT-8 and DLD1 cells were detected by western blot. Data are expressed as SD ± mean. *p < 0.05, **p < 0.01, ***p < 0.001.

Back to article page