Fig. 1: CHAF1A promotes PCNA K164 monoubiquitylation induced by UV, HU or cisplatin.
A CHAF1A-knockdown KYSE150 cells were transfected with plasmid encoding GFP-CHAF1A and then treated with HU (2 mM 4 h), UV (100 J/m2 1 h) or cisplatin (30 μM 6 h). Whole-cell lysates were analyzed using western blotting with the indicated antibodies. B CHAF1A-knockdown KYSE510 cells were treated with or without 100 J/m2 UV for 1 h. Whole-cell lysates were analyzed using western blotting with the indicated antibodies. C CHAF1A-knockdown A549 cells were treated with or without 100 J/m2 UV for 1 h. Whole-cell lysates were analyzed using western blotting with the indicated antibodies. D KYSE510 cells were transfected with control or CHAF1A siRNA oligos for 24 h and then treated with or without 100 J/m2 UV for 1 h. Whole-cell lysates were analyzed using western blotting with the indicated antibodies. E CHAF1A-knockdown KYSE510 cells were transfected with CHAF1A-GFP plasmid and treated with or without 100 J/m2 UV for 1 h. (F and G) MRC-5 cells or NE3 cells were transfected with control or CHAF1A siRNA oligos for 24 h and then treated with UV (100 J/m2 1 h), HU (2 mM 4 h) or cisplatin (30 μM 6 h). Whole-cell lysates were analyzed using western blotting with the indicated antibodies.
