Fig. 5: CHAF1A directly interacts with RAD18. | Cell Death & Disease

Fig. 5: CHAF1A directly interacts with RAD18.

From: Chromatin assembly factor 1 subunit A promotes TLS pathway by recruiting E3 ubiquitin ligase RAD18 in cancer cells

Fig. 5

A Association of endogenous CHAF1A with RAD18 in KYSE510 and A549 cells assessed by CoIP using anti-CHAF1A antibodies. B PLA assay for the interaction of CHAF1A and RAD18 in A549 cells (scale bar, 5 μm). C CHAF1A was fused to His (CHAF1A-His) and purified for His pull-down assays with GST-fused RAD18 (RAD18-GST). (D) Schematic of CHAF1A mutants. E The N-terminal (N), Middle (M) or C-terminal domain (C) of CHAF1A were fused to His and purified for His pull-down assays with GST-fused RAD18 (RAD18-GST). CBB: Coomassie brilliant blue. F KYSE510 cells were treated with or without 100 J/m2 UV for 1 h. Fluorescence microscopy was used to detect CHAF1A and RAD18 (scale bar, 5 μm), and each spot represents the number of RAD18 foci co-located with CHAF1A in an individual nucleus (n = 10). Data represent means ± SEM from three independent experiments. **P < 0.01; t-test.

Back to article page