Fig. 1: M2-TAMs infiltration is correlated with HCC resistance to sorafenib. | Cell Death & Disease

Fig. 1: M2-TAMs infiltration is correlated with HCC resistance to sorafenib.

From: Circulating mitochondrial DNA promotes M2 polarization of tumor associated macrophages and HCC resistance to sorafenib

Fig. 1

A CD163 expression in patients which responder or non-responder to sorafenib from the GSE109211 datasets. B Schematic description of sorafenib treatment in the mouse orthotopic HCC model (n = 6). C Representative images of orthotopic tumors and H&E staining in representative group (n = 6). D The ratio of mouse liver tumors to liver area in indicated groups (n = 6). E qRT-PCR analysis for the mRNA expression levels of MRC1 and ARG1 in indicated groups. F Western blotting analysis for the expression levels of CD206 in indicated groups. G, H Immunohistochemical (IHC) (G) and immunofluorescence (IF) (H) analysis for the expression levels of CD206 in representative groups. I Representative images and tumor growth curves of subcutaneous xenograft tumor model developed from HCC cells treatment with indication (n = 6). J–L The sensitivity of HCC cells (SNU739 and Hepa1-6 cells) incubated with M2-CM to sorafenib was determined by cell viability (J), cell proliferation (K), and caspase 3/7 activity assays (L). M2-CM, Conditioned medium from IL-4 and IL-13 induced M2 macrophages. ***P < 0.001.

Back to article page