Fig. 2: Conditioned medium from Sorafenib-treated HCC cells promotes M2 polarization of TAMs. | Cell Death & Disease

Fig. 2: Conditioned medium from Sorafenib-treated HCC cells promotes M2 polarization of TAMs.

From: Circulating mitochondrial DNA promotes M2 polarization of tumor associated macrophages and HCC resistance to sorafenib

Fig. 2

A Flow cytometry analysis of M2-TAM percentage in THP-1-derived macrophages treated with CM from HCC cells. B mRNA content of M2-TAM-associated cytokines in THP-1-derived macrophages and RAW264.7 cells treated with CM from HCC cells. C Immunofluorescence (IF) analysis of CD163 and CD206 in THP-1-derived macrophages and RAW264.7 cells treated with CM from HCC cells. D Flow cytometry analysis of M1-TAM contents in THP-1-derived macrophages treated with CM from HCC cells. E qRT-PCR analysis of CD86 and IL6 in THP-1-derived macrophages and RAW264.7 cells treated with CM from HCC cells. F qRT-PCR analysis for the mRNA expression of IL10, CCL2, CCL22 and VEGF in THP-1-derived macrophages and RAW264.7 which were treated with CM from HCC cells as indicated. G Transwell migration assay of THP-1-derived macrophages and RAW264.7 by CM from HCC cells as indicated. CM, Conditioned medium; SOR, Sorafenib. **P < 0.01; ***P < 0.001.

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