Fig. 3: Sorafenib promotes extracellular release of mitochondrial DNA from HCC cells by induction of apoptosis. | Cell Death & Disease

Fig. 3: Sorafenib promotes extracellular release of mitochondrial DNA from HCC cells by induction of apoptosis.

From: Circulating mitochondrial DNA promotes M2 polarization of tumor associated macrophages and HCC resistance to sorafenib

Fig. 3

A Relative mtDNA content in plasma from HCC orthotopic mouse model was detected by qPCR. B Cell free mtDNA content in supernatant of HCC cells treatment with sorafenib was quantitated via ddPCR. C The apoptosis level of HCC cells treated as indicated were detected by flow cytometry. D Relative mtDNA content in the supernatant of HCC cells treated with inhibitors of apoptosis, necroptosis and ferroptosis. E ROS in HCC cells were detected by flow cytometry. F, G The ATP levels (F) and mitochondrial membrane potential (G) of HCC cells treated as indicated were determined using a commercial kit. *P < 0.05; **P < 0.01; ***P < 0.001.

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