Fig. 4: Mitochondrial DNA promotes M2 polarization of TAMs.

A Transwell migration assay of THP-1-derived macrophages and RAW264.7 treated as indicated. B, C Western blot (B) and qRT-PCR (C) analysis the expression levels of M2-TAM marker in THP-1-derived macrophages and RAW264.7 treated as indicated. D Immunofluorescence (IF) analysis the expression levels of CD163 and CD206 in THP-1-derived macrophages and RAW264.7 treated as indicated. SOR CM, Conditioned medium from HCC cells treated with sorafenib. ***P < 0.001 versus control group; ##P < 0.01; ###P < 0.001 versus SOR CM treated group.