Fig. 6: Mitochondrial DNA promotes M2 polarization of TAMs through activating TLR9. | Cell Death & Disease

Fig. 6: Mitochondrial DNA promotes M2 polarization of TAMs through activating TLR9.

From: Circulating mitochondrial DNA promotes M2 polarization of tumor associated macrophages and HCC resistance to sorafenib

Fig. 6

A Differential mRNA expression in RNA sequencing data of mtDNA-induced M2 macrophages. B Heatmap analysis the expression of marker for M1/M2 macrophages in mtDNA induced M2 macrophages. C KEGG pathway enrichment analyses of differentially expressed genes were performed. D Western blot analysis the related protein expression levels of TLR9 pathway in THP-1-derived macrophages and RAW264.7 treated as indicated. E Immunofluorescence staining of TLR9 and CD163 in tumor tissues from patients with HCC. Scale bar: 100 μm. F, G CIBERSORT analysis the correlated between TLR9 expression and M2 macrophage infiltration level (F) or CD163 expression level (G). H Relative mtDNA content in THP-1-derived macrophages and RAW264.7 treated as indicated was detected by qPCR. I Co-localization analyses between mtDNA and TLR9 in indicated THP-1-derived macrophages and RAW264.7 cells by confocal microscopy. Scale bar: 30 μm.

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