Fig. 1: HDAC2/SIRT3 mediates the acetylation and high expression of ACSL4 in NPC.

A Heat map showed acetylated differential proteins in HK1 cells after LBH589 treatment (|Fold Change| >1.5, p < 0.05) (left) and differential proteins in normal nasopharyngeal tissues and NPC samples proteomics data IPX0001265000 (|Fold Change| > 2) (right). B Venn diagram was used to analyze the intersection of acetylated differential proteins, NPC proteomics differential proteins and KEGG ferroptosis pathway molecules. C The statistical diagram of ACSL4 acetylation by acetylation proteomics. D NPC cells were treated with LBH589 (100 nM) for 24 h, and then treated with MG-132 (20 μM) for 24 h. The acetylation of ACSL4 was detected by IP assay. After knocking down HDAC2 in NPC cells, E the acetylation of ACSL4 was detected by IP assay, F the mRNA levels of HDAC2 and SIRT1-7 were detected by qPCR, G the protein expression of HDAC2, SIRT3 and ACSL4 were detected by western blot. H NPC cells were treated with STA (20 μM) for 24 h, and the protein expression of SIRT3 and ACSL4 were detected by western blot. I The representative IHC images of HDAC2, SIRT3, and ACSL4 in 40 cases of NPC and 33 cases of normal clinical nasopharyngeal samples. J Statistical analysis of protein expression by IHC scores. K Correlation analysis for the expression of HDAC2, SIRT3, and ACSL4 by IHC scores. L After NPC cells and normal nasopharyngeal epithelial cells were treated with MG-132 (20 μM) for 24 h, IP experiments were used to detect acetylation of ACSL4. The error line is expressed as mean ± SD. No significant (ns), *** p < 0.001.