Fig. 2: ACSL4 promotes the malignant progression of NPC. | Cell Death & Disease

Fig. 2: ACSL4 promotes the malignant progression of NPC.

From: HAT1/HDAC2 mediated ACSL4 acetylation confers radiosensitivity by inducing ferroptosis in nasopharyngeal carcinoma

Fig. 2

HK1, HK1-NC, HK1-shACSL4#1, HK1-shACSL4#2, SUNE1, SUNE1-NC, SUNE1-shACSL4#1, SUNE1-shACSL4#2 and HONE1, HONE1-vec, HONE1-ACSL4-oe cells were used. A CCK8 and B colony formation assay was used to detect the cell proliferation. C Scratch assay and D Transwell assay were used to detect the cell migration and invasion. E The radioresistance of NPC cells (2 × 103 cells/well) was detected by colony formation assay under different doses (0, 1, 2, 4, 6 Gy) of IR. The 2 × 106 SUNE1-shACSL4 cells or SUNE1-NC cells were injected subcutaneously into nude mice and randomly divided into non-IR group and IR group (n = 6). F Tumor size, G tumor volume and H tumor weight was measured. I IHC was used to detect the expression of ACSL4 and Ki67 in tumor tissues. Untreated group (un), negative control (NC), blank plasmid (vec). The error line is expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.

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