Fig. 3: ACSL4 enhances radiosensitivity by inducing ferroptosis.
The stable cell lines were treated with RSL3 (5 μM) or with both RSL3 and Fer-1(2 μM) for 24 h, A CCK8 assay was used to detect the cell viability. B The statistical diagram of lip-ROS levels detected by flow cytometry. C The representative images of mitochondrial morphology were showed by TEM (red arrows indicated mitochondria with shrinkage and cristae rupture, and yellow arrows indicated normal mitochondria). D The radioresistance of NPC cells (3 × 103 cells/well) was detected by colony formation assay under different doses (0, 1, 2, 4, 6 Gy) of and IR treatment with RSL3 (5 μM). 2 × 106 SUNE1-shACSL4 or SUNE1-NC cells were injected subcutaneously into nude mice and randomly divided into non-IR group and IR group, and then divided into DMSO treatment group and RSL3 treatment group (n = 6). E Tumor size, F tumor volume and G tumor weight was measured. H IHC was used to detect the expression of ACSL4, 4-HNE and Ki67 in tumor tissues. The error line is expressed as mean ± SD. Untreated group (un), negative control (NC), blank plasmid (vec), no significant (ns), *p < 0.05, **p < 0.01, ***p < 0.001.
